《植物生理学报》 2015, 51(11): 1997-2005

灰毡毛忍冬‘渝蕾1号’内生菌对其悬浮细胞生物量及绿原酸含量的影响

李群, 汪超, 唐明, 程世君, 马丹炜, 王亚男, 卢红^*

四川师范大学生命科学学院, 成都610101

通信作者：卢；E-mail: 154194719@qq.com；Tel: 028-84480656

摘　要：

从灰毡毛忍冬‘渝蕾1号’中分离出17株内生菌, 并采用16S rDNA和18S rDNA分子鉴定结合形态特征鉴定出其中6株。将获得的6株内生菌作为诱导子添加至‘渝蕾1号’悬浮培养体系中, 探讨其对‘渝蕾1号’悬浮细胞生物量及绿原酸含量的影响。结果表明, 分离得到的6株菌分别为根瘤菌(cc2)、荧光假单孢菌(cc4)、盾壳霉菌(cc5)、高地芽孢杆菌(cc13)、同温层芽孢杆菌(cc14)及短小芽孢杆菌(cc15)。同时, 6株内生菌均不同程度地影响‘渝蕾1号’悬浮细胞生物量及次生代谢产物绿原酸含量。对生物量来说, 6种内生菌均在浓度为25 mg•L^-1时, 生物量达到最大值。对绿原酸来说, 根瘤菌、荧光假单孢菌、盾壳霉菌及同温层芽孢杆菌均在浓度为25 mg•L^-1时, 绿原酸含量达到最大, 而高地芽孢杆菌和短小芽孢杆菌在浓度为12.5 mg•L^-1时, 绿原酸含量达到最大。总结以上结果, 与对照相比, 细胞生物量和绿原酸含量会在菌株的某个浓度下大大提高, 该结果为绿原酸的工业化生产打下了基础。

关键词：灰毡毛忍冬‘渝蕾1号’; 内生菌; 生物量; 绿原酸

收稿：2015-08-14   修定：2015-10-26

资助：四川师范大学实验技术与管理项目(SYJS2014-12和SYJS2015-05)。

Effects of Endophytes of Lonicera macranthoides Cultivar ‘Yulei1’ on the Biomass Accumulation and Chlorogenic Acid Production

LI Qun, WANG Chao, TANG Ming, CHENG Shi-Jun, MA Dan-Wei, WANG Ya-Nan, LU Hong^*

College of Life Sciences, Sichuan Normal University, Chengdu 610101, China

Corresponding author: LU Hong; E-mail: 154194719@qq.com; Tel: 028-84480656

Abstract:

We isolated 17 kinds of endophytes from Lonicera macranthoides cultivar ‘Yulei1’. Besides, the 6 kinds of endophytes were identified through the 16S rDNA or 18S rDNA sequences homologous analysis, and morphological observation. This study weighed the biomass of the ‘Yulei1’ cells and analyzed the content of chlrogenic acid by the addition of different kinds of endophytes in cell suspension cultures of ‘Yulei1’. The 6 kinds of endophytes included Rhizobium sp. (cc2), Pseudomonas sp. (cc4), Coniothyrium minitans (cc5), Bacillus altitudinis (cc13), Bacillus stratosphericus (cc14), and Bacillus pumilus (cc15). In addition, the biomass accumulation and chlorogenic acid production of ‘Yulei1’ were influenced by 6 kinds of endophytes. As for biomass accumulation, the maximum accumulation of biomass was observed while 6 kinds of endophytes concentration was 25 mg•L^-1. In terms of chlorogenic acid production, the highest chlorogenic acid production of ‘Yulei1’ was tested while 4 kinds of endophytes (Rhizobium sp., Pseudomonas sp., C. minitans, and B. stratosphericus) concentration was 25 mg•L^-1. However, the highest chlorogenic acid production of ‘Yulei1’ was tested while 2 kinds of endophytes (B. altitudinis and B. pumilus) concentration was 12.5 mg•L^-1. Compared with the control, the biomass accumulation and chlorogenic acid production were greatly increased with a certain concentration. These results laid a foundation on the industrial production of chlorogenic acid.

Key words: Lonicera macranthoides cultivar ‘Yulei1’; endophytes; biomass; chlorogenic acid